EFFECT OF THE LEADER SEQUENCE ON THE EXPRESSION OF RECOMBINANT C-RUGOSA LIPASE BY SACCHAROMYCES-CEREVISIAE CELLS

Authors
FUSETTI F BROCCA S PORRO D LOTTI M
Citation
F. Fusetti et al., EFFECT OF THE LEADER SEQUENCE ON THE EXPRESSION OF RECOMBINANT C-RUGOSA LIPASE BY SACCHAROMYCES-CEREVISIAE CELLS, Biotechnology letters, 18(3), 1996, pp. 281-286
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
18
Issue
3
Year of publication
1996
Pages
281 - 286
Database
ISI
SICI code
0141-5492(1996)18:3<281:EOTLSO>2.0.ZU;2-3
Abstract
The Candida rugosa lipase I gene has been expressed in Saccharomyces c erevisiae. The recombinant lipase was efficiently synthesized only fol lowing the replacement of the enzyme endogenous leader sequence with t he signal peptide of the Kluyveromyces lactis killer toxin. Amount of accumulated lipase was about 10-20 mg/l in batch culture and over 1g/l in a computer-controlled fed-batch fermentation system.