A NEW ENZYMATIC ROUTE TO THE SYNTHESIS OF 12-KETOURSODEOXYCHOLIC ACID

Authors
BOVARA R CARREA G RIVA S SECUNDO F
Citation
R. Bovara et al., A NEW ENZYMATIC ROUTE TO THE SYNTHESIS OF 12-KETOURSODEOXYCHOLIC ACID, Biotechnology letters, 18(3), 1996, pp. 305-308
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
18
Issue
3
Year of publication
1996
Pages
305 - 308
Database
ISI
SICI code
0141-5492(1996)18:3<305:ANERTT>2.0.ZU;2-A
Abstract
Cholic acid (3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholanoic acid ) was completely and selectively transformed into 12-ketoursodeoxychol ic acid (3 alpha,7 beta-dihyrdroxy-12-oxo-5 beta-cholanoic acid) by me ans of two consecutive enzymatic steps catalyzed, the first, by 7 alph a- and 12 alpha-hydroxysteroid dehydrogenase and, the second by 7 beta -hydroxysteroid dehydrogenase. Coenzyme regeneration was carried out w ith alpha-ketoglutarate-glutamate dehydrogenase and glucose-glucose de hydrogenase, respectively.