DNA-DEGRADATION AT ELEVATED-TEMPERATURES AFTER PLASMID AMPLIFICATION IN AMINO ACID-STARVED ESCHERICHIA-COLI-CELLS

Authors
NEUBAUER P WROBEL B WEGRZYN G
Citation
P. Neubauer et al., DNA-DEGRADATION AT ELEVATED-TEMPERATURES AFTER PLASMID AMPLIFICATION IN AMINO ACID-STARVED ESCHERICHIA-COLI-CELLS, Biotechnology letters, 18(3), 1996, pp. 321-326
Citations number
24
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
18
Issue
3
Year of publication
1996
Pages
321 - 326
Database
ISI
SICI code
0141-5492(1996)18:3<321:DAEAPA>2.0.ZU;2-8
Abstract
Amino acid starvation of Escherichia coli relA mutants may be used as a method for efficient plasmid DNA amplification. Here we demonstrate. DNA degradation which occurs at elevated temperatures (42-43 degrees C) after plasmid amplification in amino acid-starved bacteria. These r esults may explain the, previously described low efficiency of plasmid DNA amplification at elevated temperatures.