VITELLOGENINS AND VITELLINS IN THE BEAN BUG, RIPTORTUS-CLAVATUS (HEMIPTERA, ALYDIDAE) - PURIFICATION, IMMUNOLOGICAL IDENTIFICATION, AND INDUCTION BY JUVENILE-HORMONE

Vitellogenins (Vgs) and vitellins (Vns) were purified from reproductiv e female adult hemolymph (HL) and newly laid eggs of the bean bug, Rip tortus clavatus. They were separated into two (Vg-1 and 2) and three ( Vn-1', Vn-1, and Vn-2) sub-components, respectively, by ion exchange c hromatography. Fused rocket immunoelectrophoresis using anti-(Vn-1) se rum showed that they are distinguished clearly into two immunologicall y distinct groups, i.e., Vg-1/Vn-1/Vn-1' and Vg-2/Vn-2. SDS-PAGE analy sis showed Vn-1 (Vn-1') and Vn-2 have the same and/or smaller subunit component polypeptides as Vg-1 and Vg-2, respectively. Vn-1' has some lower molecular weight peptides than Vn-1. Quantitative rocket immunoe lectrophoretic analysis showed that Vg-1 and Vn-1 (plus V-1') were det ected first on day 3 in the HL and day 4 in the ovary, respectively, a fter adult emergence, and increased by day 10 in non-diapause female a dults. Vg-1 synthesis is induced in diapause female adults in 1 day af ter the treatment with juvenile hormone (JH) and JH analog (JHA) or in a few days after transfer from short day to long day conditions. (C) 1996 Wiley-Liss, Inc.