VITELLOGENINS AND VITELLINS IN THE BEAN BUG, RIPTORTUS-CLAVATUS (HEMIPTERA, ALYDIDAE) - PURIFICATION, IMMUNOLOGICAL IDENTIFICATION, AND INDUCTION BY JUVENILE-HORMONE
T. Shinoda et al., VITELLOGENINS AND VITELLINS IN THE BEAN BUG, RIPTORTUS-CLAVATUS (HEMIPTERA, ALYDIDAE) - PURIFICATION, IMMUNOLOGICAL IDENTIFICATION, AND INDUCTION BY JUVENILE-HORMONE, Archives of insect biochemistry and physiology, 31(4), 1996, pp. 395-412
Vitellogenins (Vgs) and vitellins (Vns) were purified from reproductiv
e female adult hemolymph (HL) and newly laid eggs of the bean bug, Rip
tortus clavatus. They were separated into two (Vg-1 and 2) and three (
Vn-1', Vn-1, and Vn-2) sub-components, respectively, by ion exchange c
hromatography. Fused rocket immunoelectrophoresis using anti-(Vn-1) se
rum showed that they are distinguished clearly into two immunologicall
y distinct groups, i.e., Vg-1/Vn-1/Vn-1' and Vg-2/Vn-2. SDS-PAGE analy
sis showed Vn-1 (Vn-1') and Vn-2 have the same and/or smaller subunit
component polypeptides as Vg-1 and Vg-2, respectively. Vn-1' has some
lower molecular weight peptides than Vn-1. Quantitative rocket immunoe
lectrophoretic analysis showed that Vg-1 and Vn-1 (plus V-1') were det
ected first on day 3 in the HL and day 4 in the ovary, respectively, a
fter adult emergence, and increased by day 10 in non-diapause female a
dults. Vg-1 synthesis is induced in diapause female adults in 1 day af
ter the treatment with juvenile hormone (JH) and JH analog (JHA) or in
a few days after transfer from short day to long day conditions. (C)
1996 Wiley-Liss, Inc.