EFFECT OF 12 ATMOSPHERES HELIUM-OXYGEN ON THE RESPONSE OF MICE TO CONVULSANT DRUGS

The mechanism by which 12 atm abs of a helium-oxygen gas mixture (heli ox) antagonizes behavioral effects of ethanol is unknown. Although the threshold for pressure-reversal of general anesthesia and expression of the high pressure neurologic syndrome (HPNS) is well above 12 atm a bs in mice, the ethanol antagonism by 12 atm abs heliox could result f rom similar underlying excitatory effects. To investigate this possibi lity, the behavior of water-injected control mice and the latency to c onvulsions in drug-injected mice were determined in 1 atm abs air and 12 atm abs heliox. Four convulsant drugs were tested: picrotoxin (2 mg /kg), dl-allylglycine (300 mg/kg), isoniazid (300 mg/kg), and l-methio nine-dl-sulfoximine (170 mg/kg). Responses were videotaped to observe behavior and to measure latency to the onset of myoclonus and clonus. Results indicated no observable excitatory effects of 12 atm abs in co ntrol mice. The latency to myoclonus was significantly reduced by pres sure in allylglycine-treated mice but not in mice treated with the oth er convulsants. Latency to clonus was not significantly altered by pre ssure, relative to latency at 1 atm abs heliox, for any drug tested. I n conclusion, the present findings indicate that exposure to 12 atm ab s heliox is not proconvulsant and, thus, the findings do not support t he hypothesis that 12 atm abs heliox antagonizes ethanol indirectly vi a an increase in central nervous system excitability.