INCREASE OF HYBRIDOMA PRODUCTIVITY USING AN ORIGINAL DIALYSIS CULTURESYSTEM

Hybridoma cell growth and monoclonal antibody production were investig ated with a laboratory-made system in which cells were grown in dialys is tubing (MW cut-off 25 kD). The dialysis system contained 10 ml of c ell suspension and was immersed in 200 ml of culture medium which when replaced or was at 4-day intervals. With this system, monoclonal anti body concentrations similar to those observed in ascites (concentratio ns in the order of one gramme per liter) were obtained. With no medium replacement, the antibody production was 3.3 g/l and the cell product ivity 3.2 x 10(-8) mug of IgM produced per cell in one minute. With me dium replacement the antibody production was higher, 4.4 g/l but the c ell productivity was lower, 1.49 x 10(-8) mug per cell in one minute. Cells cultivated in non-optimized conditions were better producers tha n cells growing in a good environment.