G. Fertig et al., BIOTECHNOLOGICAL ASPECTS OF THE PRODUCTION OF HUMAN PRO-KALLIKREIN USING THE ACNPV-BACULOVIRUS-EXPRESSION SYSTEM, Cytotechnology, 11(1), 1993, pp. 67-75
We have investigated large scale production processes (up to 2 liters)
of recombinant proteins using the baculovirus expression system in or
der to optimize the product yields. Experiments using cell lines of Sp
odoptera frugiperda (Sf9) and Mamestra brassicae (IZD-Mb0503) were per
formed to show the different production capacities of the cell lines.
The influence of the infection at different cell densities is describe
d. Beyond that, TC100-, IPL41- and serum-free IPL41-medium were compar
ed to demonstrate their different capabilities of supporting cell grow
th and protein expression. Additionally, the inhibitory effect of FCS
on the protease activity of kallikrein, which is produced in its zymog
enic form, is discussed. Improved production parameters are described,
which enabled us to produce up to 8000 units of activated pro-kallikr
ein within 14 days using perfusion cultivation.