SPINAL-CORD MECHANISMS OF OPIOID TOLERANCE AND DEPENDENCE - FOS-LIKE IMMUNOREACTIVITY EXPRESSION INCREASES IN SUBPOPULATIONS OF SPINAL-CORDNEURONS DURING WITHDRAWAL

Citation
Ds. Rohde et al., SPINAL-CORD MECHANISMS OF OPIOID TOLERANCE AND DEPENDENCE - FOS-LIKE IMMUNOREACTIVITY EXPRESSION INCREASES IN SUBPOPULATIONS OF SPINAL-CORDNEURONS DURING WITHDRAWAL, Neuroscience, 72(1), 1996, pp. 233-242
Citations number
60
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
03064522
Volume
72
Issue
1
Year of publication
1996
Pages
233 - 242
Database
ISI
SICI code
0306-4522(1996)72:1<233:SMOOTA>2.0.ZU;2-4
Abstract
Tolerance to the analgesic effects of morphine results in part from th e development of a compensatory response in neurons that express the o pioid receptor or of neural circuits in which those neurons participat e. According to this Formulation, withdrawal from morphine results in an overshoot of several neuronal properties because of the unopposed a ction of the compensatory response system. To identify the population of spinal cord neurons that underlies this state, we monitored express ion of Fos-like immunoreactivity, after naltrexone-precipitated abstin ence in normal and morphine-tolerant rats. After daily (five days) imp lantation of morphine or placebo pellets, the rats received an injecti on of saline or naltrexone and behavior was monitored for 1 h. The rat s were then killed, their spinal cords removed and 50-mu m transverse sections of the lumbar cord were immunostained with a rabbit polyclona l antiserum directed against Fos. Naltrexone injection in the placebo group did not increase spinal cord Fos expression. Naltrexone-precipit ated abstinence resulted in an increase in Fos expression at all level s of the spinal cord; the greatest increase and densest staining was i n laminae I through VI. Importantly, when withdrawal was precipitated in anesthetized rats, we recorded a significant reduction in Fos expre ssion, particularly in laminae III through VI, but there was persisten t expression in the superficial dorsal horn, particularly in lamina I. These results suggest that spinal cord nociresponsive neurons are sen sitized during the development of tolerance. This sensitization is unm asked by the administration of naltrexone and is manifested by fos ind uction in laminae I/II in awake or anesthetized withdrawing animals. T he underlying mechanisms of tolerance development may be similar to th ose that underlie injury-induced central sensitization and hyperalgesi a.