ACIDIC PHOSPHOLIPIDS INHIBIT THE PHOSPHOLIPASE-D ACTIVITY OF RAT-BRAIN NEURONAL NUCLEI

Authors
KANFER JN MCCARTNEY DG SINGH IN FREYSZ L
Citation
Jn. Kanfer et al., ACIDIC PHOSPHOLIPIDS INHIBIT THE PHOSPHOLIPASE-D ACTIVITY OF RAT-BRAIN NEURONAL NUCLEI, FEBS letters, 383(1-2), 1996, pp. 6-8
Citations number
28
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
383
Issue
1-2
Year of publication
1996
Pages
6 - 8
Database
ISI
SICI code
0014-5793(1996)383:1-2<6:APITPA>2.0.ZU;2-N
Abstract
An oleate dependent form of phospholipase D is present in rat brain ne uronal nuclei and both the hydrolytic and transphosphatidylation activ ities measured. Several acidic phospholipids were found to inhibit thi s activity in a dose dependent manner. The IC50 values varied from 3.5 mu M for PIP2 to 200 mu M for phosphatidic acid. The hydrolysis of PI P2 by phospholipase C would be expected to result in the disinhibition of the oleate dependent phospholipase D activity.