H. Kayser et H. Meisel, STIMULATION OF HUMAN PERIPHERAL-BLOOD LYMPHOCYTES BY BIOACTIVE PEPTIDES DERIVED FROM BOVINE-MILK PROTEINS, FEBS letters, 383(1-2), 1996, pp. 18-20
The in vitro modulation of the proliferation of human peripheral blood
lymphocytes by different synthetic peptides derived from milk protein
s was investigated. Therefore, proliferation changes were followed up
after incorporation of BrdU into the DNA, and the influence on protein
biosynthesis was measured using the [H-3]leucine incorporation test.
Tyr-Gly and Tyr-Gly-Gly significantly enhanced (maximal 90 and 35%, re
spectively) the proliferation of PBL. For beta-casomorpbin-7 and beta-
casokinin-10, lymphocyte proliferation was suppressed at lower concent
rations, but stimulated at higher concentrations (greater than or equa
l to 10(-7) mol/l). Protein synthesis was stimulated (maxima at 25%) o
nly with TSr-Gly and Tyr-Gly-Gly. The findings point to a need for fur
ther studies on the possible function of peptides derived from milli p
roteins as orally bioavailable immunopotentiatory compounds.