CHARACTERIZATION OF THE DYSTROPHIN-SYNTROPHIN INTERACTION USING THE 2-HYBRID SYSTEM IN YEAST

The carboxy-terminal region of dystrophin has previously been shown to interact directly with alpha 1 syntrophin, a cytoplasmic component of the dystrophin-glycoprotein complex, by in vitro biochemical studies such as overlay assay or immunoprecipitation. Using the two-hybrid sys tem, we have isolated from a human heart cDNA library the entire codin g sequence of human al syntrophin, therefore confirming for the first time this interaction via an in vivo approach. In addition, we have re duced the interaction domain to the distal half of oil syntrophin.