A. Castello et al., CHARACTERIZATION OF THE DYSTROPHIN-SYNTROPHIN INTERACTION USING THE 2-HYBRID SYSTEM IN YEAST, FEBS letters, 383(1-2), 1996, pp. 124-128
The carboxy-terminal region of dystrophin has previously been shown to
interact directly with alpha 1 syntrophin, a cytoplasmic component of
the dystrophin-glycoprotein complex, by in vitro biochemical studies
such as overlay assay or immunoprecipitation. Using the two-hybrid sys
tem, we have isolated from a human heart cDNA library the entire codin
g sequence of human al syntrophin, therefore confirming for the first
time this interaction via an in vivo approach. In addition, we have re
duced the interaction domain to the distal half of oil syntrophin.