CHEMOENZYMATIC SYNTHESIS OF ETHYL O-(BETA-D-GALACTOPYRANOSYL)O-BETA-D-GLYCOPYRANOSYL DISACCHARIDES USING THE BETA-GALACTOSIDASE FROM BACILLUS-CIRCULANS

Authors
VIC G HASTINGS JJ HOWARTH OW CROUT DH
Citation
G. Vic et al., CHEMOENZYMATIC SYNTHESIS OF ETHYL O-(BETA-D-GALACTOPYRANOSYL)O-BETA-D-GLYCOPYRANOSYL DISACCHARIDES USING THE BETA-GALACTOSIDASE FROM BACILLUS-CIRCULANS, Tetrahedron : asymmetry, 7(3), 1996, pp. 709-720
Citations number
33
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Inorganic & Nuclear","Chemistry Physical
Journal title
Tetrahedron : asymmetry → ACNP
ISSN journal
09574166
Volume
7
Issue
3
Year of publication
1996
Pages
709 - 720
Database
ISI
SICI code
0957-4166(1996)7:3<709:CSOEO>2.0.ZU;2-L
Abstract
Different ethyl 1-thio-beta-D-disaccharides have been synthesised by t ransgalactosylation using the beta-galactosidase from Bacillus circula ns as biocatalyst. This beta-galactosidase shows mainly a beta-1-4 spe cificity in the galactosyl transfer. Gal-beta-(1-4)-O-beta-D-GlcSEt 15 was obtained in 36% yield, Gal-beta-(1-4)-O-alpha-D-GlcSEt 19 in 30% yield, Gal-beta-(1-4)-O-beta-D-GalSEt 17 in 60% yield, Gal-beta-(1-4)- O-beta-D-GalNAcSEt 20 in 49% yield, a-(1-4)-O-beta-D-Gal-beta-(1-4)-O- beta-D-GalNAcSEt 21 in 9% yield, Gal-beta-(1-6)-O-beta-D-GlcSEt 16 in 3% yield and Gal-beta-(1-3)-O-beta-D-XylSEt 25% yield. (C) 1996 Elsevi er Science Ltd