A sensitive fluorometric method for assaying malarial pigment, haemozo
in, has been developed and used to determine the haemozoin content of
blood and tissue samples. PIasmodium falciparum rings and trophozoites
were found to contain 23 and 339 ng haemozoin/10(6) parasitized red b
lood cells (PRBCs), respectively. Unsynchronized Plasmodium berghei NK
65 or ANKA parasites from infected mice contained 27 and 61 ng haemozo
in/10(6) PRBCs, respectively. An exponential accumulation of haemozoin
within 18 days after infection was demonstrated in liver and spleen t
issue, representing up to 0.2% of the tissue by wet weight by day 18.
Histology indicated that the accumulation occurred predominantly in th
e tissue monocytes. In the brain, the levels of haemozoin after 8 days
of infection were considerably lower than they were in the liver or s
pleen, and most of the pigment appeared to be that present inside para
sitized red blood cells. CBA/Ca mice infected with P. berghei ANKA (a
cerebral malaria model) had significantly higher amounts of haemozoin
in the brain than did ICR mice infected with P. berghei NK65. Thus, ha
emozoin levels in tissue increase with the duration of infection, and
its presence may be associated with cerebral pathology.