EVALUATION OF CYCLIC-AMP ACCUMULATION IN EBV-TRANSFORMED HUMAN B-LYMPHOCYTES - EFFECTS OF DOPAMINE AGONISTS, ISOPROTERENOL, PROSTAGLANDIN E(1), CHOLERA-TOXIN, FORSKOLIN, AND PHORBOL 12-MYRISTATE-13-ACETATE

1. Phorbol 12-myristate-13-acetate (PMA), a protein kinase C activator , elevated cyclic AMP accumulation in EBV-transformed human B-lymphocy tes, and potentiated isoproterenol-, prostaglandin- (PGE(1)), cholera toxin-, and forskolin-stimulated cyclic AMP accumulation. 2. The dopam ine D-1 receptor agonist, SXF38393 (10(-7) to 10(-5) M), had no effect on cyclic AMP accumulation in transformed human B-lymphocytes. 3. The dopamine D-2 receptor agonist, quinpirole (10(-7) to 10(-4) M) did no t inhibit cyclic AMP accumulation even when cyclic AMP accumulation wa s maximized by the addition of PMA and forskolin. 4. These data sugges t that dopamine D-1- and D-2-receptor coupling to a cyclic AMP generat ing system is not present at detectable levels in transformed human B- lymphocytes.