INFLUENCE OF TOOTH CRYOPRESERVATION AND STORAGE TIME ON MICROLEAKAGE

Objectives. This study was conducted to compare microleakage of two ne w dentin bonding agents on freshly extracted teeth, cryopreserved teet h, or teeth stored in water containing 0.5% chloramine at 4 degrees C. Methods. Rectangular Class V cavity preparations were made on the buc cal and the lingual surface of wisdom teeth. They were filled with eit her Scotchbond Multi-Purpose and Z100 (3M Dental Products) or with Glu ma 2000 and Pekafill (Bayer Dental). After thermocycling, silver stain ing penetration was evaluated under a light microscope. SEM examinatio n and EDX analysis were performed to evaluate the microleakage pattern . The results were analyzed by the use of a two-way analysis of varian ce. Results. Cryopreservation for 13 wk or 12 d refigeration did not p roduce changes in the amount of microleakage. However; 48 d or longer of refrigeration increased microleakage. There was no correlation betw een changes in microleakage and storage time. Specimens prepared with both dentin bonding agents exhibited the same microleakage values and the same microleakage pattern. Significance. Refrigeration at 4 degree s C in 0.5% chloramine for 48 d or longer may cause an increase in mic roleakage. Cryopreservation for 13 wk or short-term refrigeration did not affect the microleakage.