COMPARATIVE-EVALUATION OF MICROPLATE ENZYME-LINKED-IMMUNOSORBENT-ASSAY VERSUS PLAQUE REDUCTION ASSAY FOR ANTIVIRAL SUSCEPTIBILITY TESTING OF HERPES-SIMPLEX VIRUS ISOLATES

Authors
SAFRIN S PALACIOS E LEAHY BJ
Citation
S. Safrin et al., COMPARATIVE-EVALUATION OF MICROPLATE ENZYME-LINKED-IMMUNOSORBENT-ASSAY VERSUS PLAQUE REDUCTION ASSAY FOR ANTIVIRAL SUSCEPTIBILITY TESTING OF HERPES-SIMPLEX VIRUS ISOLATES, Antimicrobial agents and chemotherapy, 40(4), 1996, pp. 1017-1019
Citations number
10
Categorie Soggetti
Pharmacology & Pharmacy",Microbiology
Journal title
Antimicrobial agents and chemotherapy → ACNP
ISSN journal
00664804
Volume
40
Issue
4
Year of publication
1996
Pages
1017 - 1019
Database
ISI
SICI code
0066-4804(1996)40:4<1017:COME>2.0.ZU;2-#
Abstract
We tested the antiviral susceptibilities of 30 clinical isolates of he rpes simplex virus using the microplate in situ enzyme-linked immunoso rbent assay (MISE) and the plaque reduction assay (PRA). There was con cordance for 26 of 30 acyclovir results and all 30 foscarnet results. MISE and PRA results each predicted the response to acyclovir in 12 of 14 instances and the response to foscarnet in 8 instances. MISE is mo re rapid than PRA, has an objective endpoint, and correlates well with the clinical response to therapy.