PAF AND HEMATOPOIESIS .10. MACROPHAGE-COLONY-STIMULATING FACTOR AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR ENHANCE PLATELET-ACTIVATING-FACTOR ACETYLHYDROLASE PRODUCTION BY HUMAN BLOOD-DERIVED MACROPHAGES
F. Dupuis et al., PAF AND HEMATOPOIESIS .10. MACROPHAGE-COLONY-STIMULATING FACTOR AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR ENHANCE PLATELET-ACTIVATING-FACTOR ACETYLHYDROLASE PRODUCTION BY HUMAN BLOOD-DERIVED MACROPHAGES, Biochimica et biophysica acta. Molecular cell research, 1311(1), 1996, pp. 27-32
Platelet-activating factor (PAF), a phospholipid autacoid with potent
regulatory functions, is synthesized by stimulated monocytes. Macropha
ges are a source of the plasma acetylhydrolase activity (AHA) which re
gulates PAF concentrations. Granulocyte-macrophage colony-stimulating
factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF) are i
nvolved in the differentiation and functions of cells from the monocyt
ic/macrophagic lineage. This work reports that M-CSF and GM-CSF stimul
ated AHA production by human blood monocyte-derived macrophages in a t
ime- and dose-dependent manner. After 7 days of culture without serum,
a 6- and 4-fold increase was found in cells treated with M-CSF (1000
U/ml) and CM-CSF (50 ng/ml), respectively. M-CSF (up to 1000 U/ml) and
GM-CSF (up to 10 ng/ml) did not induce PAF production by human blood
monocytes. While GM-CSF (10 ng/ml) and interleukin-1 (10 U/ml) stimula
ted M-CSF production from monocyte-derived macrophages, PAF did not. T
hese results indicate that M-CSF and GM-CSF enhance AHA production by
human blood-derived macrophages cultured in low serum concentrations.
Clearly the effects of growth factors on AHA production in vivo deserv
e to be assessed.