LOSS OF THE CHROMOSOMAL REGION 5Q11-Q31 IN THE MYELOID CELL-LINE HL-60 - CHARACTERIZATION BY COMPARATIVE GENOMIC HYBRIDIZATION AND FLUORESCENCE IN-SITU HYBRIDIZATION

Comparative genomic hybridization was used to identify the regions of genomic gain and loss in the myeloid cell line HL-60. These included a mplification at 8q24 corresponding to previous reports of overrepresen tation of the MYC gene; loss of material from the short arms of chromo somes 9 (9p21-p23), 10, and 17; loss of the chromosome regions 9q32-qt er and 14q11-q24; and an extra copy of chromosome 18. Additionally, de letion of the 5q11-q31 region was noted and was associated with transl ocation of chromosome 5 material to chromosomes 16 and a dic(5;17)(q11 ;p11) chromosome (previously described as mar3). Loss of chromosome 5 material in myeloid malignancies, including the M2 subtype from which HL-60 was derived, is usually associated with interstitial deletions o f the long arm, including the critical 5q31 region, resulting in a 5q- chromosome. The HL-60 cell line may be a useful model to investigate the role of potential tumour suppressor genes associated with loss of 5q material in myeloid leukaemias. (C) 1996 Wiley-Liss, Inc.