Erm. Wickremesinhe et Rn. Arteca, HPLC SEPARATION OF CEPHALOTAXINE, HARRINGTONINE AND HOMOHARRINGTONINEFROM CALLUS AND ROOT CULTURES OF CEPHALOTAXUS-HARRINGTONIA, Journal of liquid chromatography & related technologies, 19(6), 1996, pp. 889-897
Citations number
17
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A simple extraction and analytical protocol was developed to assay cep
halotaxine, harringtonine, and homoharringtonine from callus and root
cultures of Cephalotaxus harringtonia. The process involves extraction
by methanol followed by partitioning between 0.5% ammonium hydroxide
and chloroform. The chloroform fraction recovered greater than 90% of
all three alkaloids. This fraction was concentrated to dryness, resusp
ended in methanol and analyzed by high pressure liquid chromatography
using a UV detector. Peaks corresponding to all three alkaloids were i
dentified by comparing their retention times with authentic standards
and their identity confirmed by fast atom bombardment spectrometry. Th
e root cultures contained higher levels of harringtonine and homoharri
ngtonine (2.4 and 3.9 mg/kg dry matter, respectively) compared to the
callus cultures; however, the levels of cephalotaxine were comparable
(10.2 mg/kg dry matter).