HPLC METHOD FOR THE DETERMINATION OF KETOROLAC IN HUMAN PLASMA

A precise and accurate HPLC method for the quantification of ketorolac in human plasma is described. Ketorolac and an added internal standar d (a fluoro analog of ketorolac) are extracted from acidified plasma s amples and the extract is further purified. The reconstituted sample i s injected onto a reverse phase HPLC column. The method has a linear c oncentration range of 0.010-3.00 mu g of ketorolac per aliquot of plas ma, using 0.1 to 1.0 mL of plasma for analysis. The quantification lim it of the method is 0.010 mu g/mL. The intra- and inter-assay % CVs we re less than 5.5%, and the mean recoveries ranged from 96% to 109%. Th e absolute recovery was 92%.