ISOLATION AND COMPLETE SEPARATION OF LIPIDS FROM NATURAL SOURCES

A method of isolation and complete separation of lipids from natural s ources into classes and species is reported which combines our previou sly published techniques with new techniques described in this article for the first time. Pigments are separated from crude total lipid ext racts with two successive TLC systems: a) petroleum ether/benzene/glac ial acetic acid (30:70:2) and b) acetone/methanol/water (40:20:1). Pig ment-free total lipids are separated on a silicic acid column into neu tral, glyco- and phospholipids. Neutral, glyco- and phospholipids are separated into classes and species by suitable HPLC methods.