DETERMINATION OF PIPERACILLIN IN BIOLOGICAL SAMPLES BY HPLC

A rapid, specific and reliable technique has been developed for the de termination of piperacillin in biological samples by High Performance Liquid Chromatography. To do so, reverse phase liquid chromatography w as used, employing acetonitrile-phosphate buffer 0,1M and pH=6 as the mobile phase (20:80 v/v) and a detector wavelength of 254 nm. Concentr ations ranged between 0,5 mu g/mL, and 200 mu g/mL, divided into two c alibrations: high concentrations, 15 mu g/mL to 200 mu g/mL, and low c oncentrations, 0,5 mu g/mL to 15 mu g/mL. The intraday and interday re producibility of the analytical technique was studied by calculating t he variation coefficient of 5 samples analyzed each day and on five co nsecutive days by ANOVA. The coefficients obtained were 4,02% and 7,11 %, respectively, for concentrations ranging between 15-200 mu g/mL and 5.71% and 7,54% for concentrations between 0,5 and 15 mu g/mL.