Je. Haky et al., PERFLUOROOCTYL AND PERFLUOROBUTYL BONDED ALUMINA STATIONARY PHASES FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of liquid chromatography & related technologies, 19(2), 1996, pp. 307-332
Citations number
32
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The preparation and properties of perfluorooctylalumina (PFOA) and per
fluorobutylalumina (PFBA) high performance liquid chromatographic stat
ionary phases have been investigated. The PFOA phase was produced by c
hemisorption of perfluorooctanoic acid onto the surface of alumina. Th
e PFBA phase was produced by a similar adsorption of perfluorobutylpho
sphonic acid onto alumina. Both phases exhibit reverse phase liquid ch
romatographic properties. Elemental analyses of these materials indica
ted that alkyl group surface coverage of the PFBA phase is higher than
that of the PFOA phase. In contrast, retention of solutes on the PFBA
phase is lower than that of PFOA. Isocratic capacity factors of over
20 compounds on the PFOA and PFBA phases were determined and compared
with those obtained on octadecylalumina (ODA) and octadecylsilica (ODS
) phases. In contrast to the greater retention of phenols than other c
ompounds that was evident on the unfluorinated ODA phase, the retentio
n of phenols on the PFOA and PFBA phases was not found to be significa
ntly different from that of other compounds. These results are attribu
ted to a reduced degree of hydrogen bonding interactions between pheno
lic solutes and the PFOA and PFBA phases compared to those which occur
between phenols and the ODA phase. preliminary investigations of the
utilization of the PFOA phase for the separation of peptides and the e
mployment of the PFBA phase for the rapid separation of phenols are al
so described.