COMPARISON OF 3 METHODS FOR COMPETITIVE-BINDING OF MONOCLONAL-ANTIBODIES - THE LOCALIZATION OF ANTIGENIC SITES FOR MONOCLONAL-ANTIBODIES ONPANULIRUS-INTERRUPTUS HEMOCYANIN

The competitive binding of a panel of monoclonal antibodies against he mocyanin of Panulirus interruptus hemocyanin was investigated with thr ee different methods, A competitive-binding immunoassay method was mor e successful in the determination of ternary complexes than gel electr ophoresis and gel filtration. The latter two methods could only be app lied with antibodies possessing a higher affinity, Eleven monoclonal a ntibodies were assigned to groups on the basis of their interactions w ith five antigenic regions.