CYTOGENETIC CHARACTERIZATION OF THE HUMAN PROSTATE-CANCER CELL-LINE P69SV40T AND ITS NOVEL TUMORIGENIC SUBLINES M2182 AND M15

Cytogenetic studies of a SV40T antigen immortalized human prostate epi thelial cell line (P69SV40T) and its increasingly tumorigenic tumor su blines, designated M2182 and M15, were done with GTG-banding and multi color fluorescence in situ hybridization (FISH). The parental line and each of the two sublines were near-diploid and contained several cons istent abnormalities. Two structural chromosome anomalies were noted i n all three lines; a der(7)t(5;20;7) and a der(5)t(5;9). Abnormalities that were acquired and retained in the tumor sublines after in vivo a nd/or in vitro selection included a der(1)t(1;8), der(3)t(3;14), der(2 0)t(7;20), and der(X)t(X;11). Findings unique to subline M2182 were a der(11)t(5;11) and -14. Those unique to M15 were a der(16)t(16;19) and -Y. Chromosome imbalances resulting from numerical and/or structural abnormalities in the tumor sublines involved several chromosome region s that have previously been implicated in human prostate cancer; such as loss of Xp, Y, 3p (M2182 and M15), 16q (M15), and gains for 5q (M21 82) and 8q (M2182 and M15). Collectively, the characterization of thes e lines should assist with the localization of chromosome regions, and possibly genes, that are important in the development and progression of human prostate cancer.