NF-KAPPA-B ACTIVATION IS DELAYED IN MOUSE L929 CELLS INFECTED WITH INTERFERON SUPPRESSING, BUT NOT INDUCING, VESICULAR STOMATITIS-VIRUS STRAINS

Vesicular stomatitis virus (VSV) mutant T1026R1 of the Indiana (IN) se rotype is a good inducer of interferon (IFN). This mutant was used to study the activation of NF-kappa B, a transcription factor necessary f or IFN induction, in mouse L929 cells that were stably transfected wit h a chimeric gene containing the human IFN-beta gene promoter attached to the chloramphenicol acetyltransferase (CAT) coding sequence. NF-ka ppa B DNA binding activity was detected as early as 30 min after virus adsorption in nuclear extracts, increased up to 4 hr, and then remain ed constant for at least 6 additional hr. The kinetics of CAT expressi on correlated with the kinetics of NF-kappa B nuclear DNA binding acti vity. Virus entry and delivery of viral components into the cytoplasm were required for NF-kappa B activation. Exposure of T1026R1 to one hi t of UV irradiation nearly completely reduced NF-kappa B activation. I n cells infected with wild-type (wt) VSV (IN), a noninducer of IFN, NF -kappa B DNA binding activity in the nucleus was delayed for several h ours after virus adsorption. Coinfection of wt VSV and T1026R1 resulte d in the reduction of T1026R1-promoted NF-kappa B activation. This inh ibitory activity of wt VSV was abolished by one hit of UV irradiation. Under similar conditions expression of the CAT gene was more UV resis tant, suggesting that IFN gene expression is regulated at multiple lev els. (C) 1996 Academic Press, Inc.