PRODUCTION OF ACYLATED ANTHOCYANINS BY BLUE FLOWERS OF HYACINTHUS-ORIENTALIS REGENERATED IN-VITRO

Acylated anthocyanins were produced by flowers of Hyacinthus orientali s that had been regenerated in vitro. The anthocyanin production was a ffected by the concentration of gibberellic acid (GA(3)) and sucrose i n Murashige and Skoog's (MS) medium and by temperature. The highest co ncentration of anthocyanin was obtained when the regenerated flowers w ere cultured for three weeks at 15 degrees on MS medium containing 1 m gl(-1) GA(3) and 30 gl(-1) sucrose. The concentration of anthocyanin i n the regenerated flowers was ca 1.2 times higher than that in held-gr own flowers. The anthocyanins in the regenerated flowers were compared with those in held-grown flowers by chromatographic and spectroscopic methods, showing that these flowers produce almost the same anthocyan ins with a little difference in composition.