SEQUENCING A COSMID CLONE OF SACCHAROMYCES-CEREVISIAE CHROMOSOME-XIV REVEALS 12 NEW OPEN READING FRAMES (ORFS) AND AN ANCIENT DUPLICATION OF 6 ORFS

A sequence of 31431 bp located on the left arm of chromosome (chr.) XI V from Saccharomyces cerevisiae was analysed. A total of 18 open readi ng frames (ORFs) could be identified. Twelve ORFs are new, two of whic h are most likely ribosomal protein genes, leaving ten ORFs of unknown function. Nine of the 18 ORFs show either at least 20% overall amino acid identity or significant regional homology to other S. cerevisiae ORFs. Additionally, six of these nine ORFs have homologues of similar size and the same transcriptional orientation within a stretch of 50 k b on chromosome IX. The degree of homology ranges from 90% overall ide ntity to 23% in 375 amino acids. The homologues on chromosome IX are g rouped in two blocks that are separated by relatively long ORFs. This is the first example of a multi-gene duplication in S. cerevisiae not linked to a centromere or subtelomere region. The sequence has been de posited in the EMBL data library under Accession Number X86470.