THE SEQUENCE OF A 21-CENTER-DOT-3 KB DNA FRAGMENT FROM THE LEFT ARM OF YEAST CHROMOSOME-XIV REVEALS LEU4, MET4, POL1, RAS2, AND 6 NEW OPEN READING FRAMES

The nucleotide sequence of a fragment of 21 308 bp from the left arm o f Saccharomyces cerevisiae chromosome XIV has been determined. Analysi s of the sequence revealed 13 open reading frames (ORFs) longer than 3 00 bp, four of which correspond to the previously identified genes LEU 4, MET4, POL1 and RAS2. One putative protein, N2160, shares considerab le homology (32% identity) with a hypothetical protein encoded by a ge ne located on chromosome XV as well as with human OCRL protein (36% id entity), involved in Lowe's syndrome. N2185 contains ten predicted tra nsmembrane segments and is similar to another putative protein (YKL146 ) from yeast. The sequence of the reported DNA fragment has been submi tted to the EMBL data library under Accession Number Z50161.