DIFFERENTIAL INDUCTION AND ACCUMULATION OF BETA-1,3-GLUCANASE AND CHITINASE ISOFORMS IN SOYBEAN HYPOCOTYLS AND LEAVES AFTER COMPATIBLE AND INCOMPATIBLE INFECTION WITH PHYTOPHTHORA-MEGASPERMA F-SP GLYCINEA
Sy. Yi et Bk. Hwang, DIFFERENTIAL INDUCTION AND ACCUMULATION OF BETA-1,3-GLUCANASE AND CHITINASE ISOFORMS IN SOYBEAN HYPOCOTYLS AND LEAVES AFTER COMPATIBLE AND INCOMPATIBLE INFECTION WITH PHYTOPHTHORA-MEGASPERMA F-SP GLYCINEA, Physiological and molecular plant pathology, 48(3), 1996, pp. 179-192
Phytophthora megasperma f.sp. glycinea infection greatly induced the s
ynthesis and accumulation of beta-1,3-glucanases and chitinases in hyp
ocotyls and leaves of soybean seedlings. Native polyacrylamide gel ele
ctrophoresis and isoelectric focusing were used to identify and quanti
fy the isoforms of the two hydrolases in soybean tissues. Some beta-1,
3-glucanase isoforms were constitutively expressed in soybeans, but ot
her isoforms only accumulated in soybean or only in a particular organ
of soybean after inoculation with P. m. f.sp. glycinea infection or a
fter treatment with mercuric chloride. The pI 8 . 4 isoform, Gb 2 of b
eta-1,3-glucanase constitutively present in soybean hypocotyls and lea
ves completely disappeared after mercuric chloride treatment. P. m. f.
sp. glycinea infection and mercuric chloride treatment leads to the ac
cumulation of distinctly different isoforms of chitinase in soybean ti
ssues. The acidic chitinase isoform Ca 4 was induced and accumulated i
n hypocotyls, bur not in leaves, after pathogen infection and mercuric
chloride treatment. In general, beta-1,3-glucanase and chitinase isof
orms accumulated in the diseased soybean tissues during symptom develo
pment in both compatible and incompatible interactions. The results al
so suggest that pathogen infection and chemical stress may function in
a different organ-specific manner to induce isoforms of beta-1,3-gluc
anase and chitinase in soybeans. (C) 1996 Academic Press Limited.