DETERMINATION OF STABILITY OF BRUCELLA-ABORTUS RB51 BY USE OF GENOMICFINGERPRINT, OXIDATIVE-METABOLISM, AND COLONIAL MORPHOLOGY AND DIFFERENTIATION OF STRAIN RB51 FROM B-ABORTUS ISOLATES FROM BISON AND ELK
Ae. Jensen et al., DETERMINATION OF STABILITY OF BRUCELLA-ABORTUS RB51 BY USE OF GENOMICFINGERPRINT, OXIDATIVE-METABOLISM, AND COLONIAL MORPHOLOGY AND DIFFERENTIATION OF STRAIN RB51 FROM B-ABORTUS ISOLATES FROM BISON AND ELK, Journal of clinical microbiology, 34(3), 1996, pp. 628-633
Brucella abortus RB51 and isolates from cattle, bison, and elk were ch
aracterized by pulsed-field gel electrophoresis and standard technique
s for biotyping Brucella species, which included biochemical, morpholo
gical, and antigenic techniques, phage susceptibility, and antibiotic
resistance. The objectives were to ascertain the stability of RB51 and
to differentiate RB51 from other brucellae. Genomic restriction endon
uclease patterns produced by pulsed-field gel electrophoresis demonstr
ated a unique fingerprint for RB51 relative to other brucellae. Compar
isons of the oxidative metabolic profiles of RB51 after time in vivo (
14 weeks) and in vitro (75 passages) showed no change in characteristi
c patterns of oxygen uptake on selected amino acid and carbohydrate su
bstrates, Strain RB51 was biotyped as a typical rough B. abortus biova
r 1 (not strain 19) after animal passage or a high number of passages
in vitro and remained resistant to rifampin or penicillin and suscepti
ble to tetracycline, No reactions with A or M antiserum or with a mono
clonal antibody to the O antigen of Brucella lipopolysaccharides were
detected; however, RB51 agglutinated with R antiserum. The results ind
icate that the genomic fingerprint and rough colonial morphology of RB
51 are stable characteristics and can be used to differentiate this va
ccine strain from Brucella isolates from cattle, bison, and elk.