IMMUNODETECTION OF PNEUMOCYSTIS-CARINII IN BRONCHOALVEOLAR LAVAGE SPECIMENS COMPARED WITH METHENAMINE SILVER STAIN

Authors
LAUTENSCHLAGER I LYYTIKAINEN O JOKIPII L JOKIPII A MAICHE A RUUTU T TUKIAINEN P RUUTU P
Citation
I. Lautenschlager et al., IMMUNODETECTION OF PNEUMOCYSTIS-CARINII IN BRONCHOALVEOLAR LAVAGE SPECIMENS COMPARED WITH METHENAMINE SILVER STAIN, Journal of clinical microbiology, 34(3), 1996, pp. 728-730
Citations number
18
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
34
Issue
3
Year of publication
1996
Pages
728 - 730
Database
ISI
SICI code
0095-1137(1996)34:3<728:IOPIBL>2.0.ZU;2-J
Abstract
Immunodetection of Pneumocystis carinii, based on immunofluorescence a nd use of a monoclonal antibody specific for an antigen located within the cyst wall and detectable after trypsin digestion only, was compar ed with a methenamine silver stain in 553 bronchoalveolar lavage speci mens from immunosuppressed patients. P. carinii was found by immunoflu orescence in 72 (86%) and by silver stain in 68 (81%) of the total of 84 positive samples detected by either or both of theses methods.