COMPREHENSIVE STUDY OF SEVERAL GENERAL AND TYPE-SPECIFIC PRIMER PAIRSFOR DETECTION OF HUMAN PAPILLOMAVIRUS DNA BY PCR IN PARAFFIN-EMBEDDEDCERVICAL CARCINOMAS

Authors
BAAY MFD QUINT WGV KOUDSTAAL J HOLLEMA H DUK JM BURGER MPM STOLZ E HERBRINK P
Citation
Mfd. Baay et al., COMPREHENSIVE STUDY OF SEVERAL GENERAL AND TYPE-SPECIFIC PRIMER PAIRSFOR DETECTION OF HUMAN PAPILLOMAVIRUS DNA BY PCR IN PARAFFIN-EMBEDDEDCERVICAL CARCINOMAS, Journal of clinical microbiology, 34(3), 1996, pp. 745-747
Citations number
20
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
34
Issue
3
Year of publication
1996
Pages
745 - 747
Database
ISI
SICI code
0095-1137(1996)34:3<745:CSOSGA>2.0.ZU;2-9
Abstract
We have compared the efficacies of three general primer pairs for the detection of human papillomavirus (HPV) DNA in formaldehyde-fixed para ffin-embedded carcinomas. The use of these primer pairs leads to under estimates of the HPV prevalence (BP5/6, 61.1%; CPI/IIG, 57.4%; MY09/11 , 46.9%; combined, 72.8%). The efficacy of each primer pair seemed to be inversely correlated to the length of the amplimer produced. By usi ng newly developed type-specific primer pairs (amplimer length, approx imately 100 bp), an increase in HPV DNA detection (87.6%) was found.