VIABLE(TM) AC-2, A NEW ADULT BOVINE SERUM-BASED AND COLOSTRUM-BASED SUPPLEMENT FOR THE CULTURE OF MAMMALIAN-CELLS

In this study we have shown that Viable(TM) AC-2, a medium based on an ultrafiltrate fraction of bovine colostrum and adult bovine serum, ca n be used successfully as a fetal bovine serum (FBS) substitute in the culture of several anchorage-dependent and independent cell lines. Of the 15 cell lines cultured in 8% Viable AC-2 in microplates, 10 reach ed the maximum cell density of 65%-123% of that in 10% FBS, 4 cell lin es reached maximum cell density of 35%-65% of that in 10% FBS and only one cell fine, a human osteosarcoma G-292, grew slowly in Viable AC-2 . In a small-scale suspension culture, 8%-15% Viable AC-2 supports the growth of Chinese hamster ovary cells (CHO-K1) on microcarriers in sp inner flasks significantly better than 10% FBS. Shionogi mouse mammary turner cell line (S115) transfected with human ai-adrenergic receptor subtype C2 was used as a model to study recombinant protein productio n in Viable AC-2-supplemented medium. The results showed that in cell culture flasks and in an ACUSYST-R(TM) bioreactor the alpha(2)-C2 rece ptor expression level per mg of total protein was similar in both Viab le AC-2 and FBS.