EXTRACELLULAR POLYSACCHARIDES OF CANDIDA-MUCIFERA STRAIN CCY-29-170-1

Extracellular polysaccharides produced by Candida mucifera strain CCY 29-170-1 in a liquid medium containing malt extract have been investig ated by chemical, enzymic, and NMR spectroscopic methods. Mild acid hy drolysis of the native polysaccharide, followed by enzymic treatments of the polymeric residue, afforded two low-molecular neutral homopolys accharides. The dominant one was a (1 --> 4)-alpha-D-glucan with appro ximately each fifth chain unit branched on O-6. The other polysacchari de was a linear (1 --> 4)-beta-D-xylan. The results of linkage analysi s evidenced that, originally, some xylan-chain units bore on O-3 subst ituents, which were split off during the hydrolytic steps.