CLYCOSIDE AND POLYSACCHARIDE HYDROLASE ACTIVITY OF THE RUMEN ANAEROBIC FUNGUS CAECOMYCES COMMUNIS (SPHAEROMONAS-COMMUNIS SENSU-ORPIN) AT EARLY AND FINAL STAGES OF THE DEVELOPMENTAL CYCLE

The rumen anaerobic fungus Caecomyces communis was grown in a fermente r in Lowe medium. We studied four polysaccharide hydrolases and three glycoside hydrolases at early and final stages. We found a difference in cell association for these enzymes depending on the developmental s tage. The endocellulase and beta-D-fucosidase were early synthesized, and their activities decreased at the end of the developmental cycle. On the contrary, the beta-D-glucosidase, beta-D-xylosidase and xylanas e activities increased during the cycle. The avicelase and the CM-cell ulase activities linked with thalli increased, whereas the extracellul ar activities of these enzymes decreased.