GLYCEROL DEHYDRATASE ACTIVITY - THE LIMITING STEP FOR 1,3-PROPANEDIOLPRODUCTION BY CLOSTRIBIUM-BUTYRICUM DSM-5431

Citation
S. Abbadandaloussi et al., GLYCEROL DEHYDRATASE ACTIVITY - THE LIMITING STEP FOR 1,3-PROPANEDIOLPRODUCTION BY CLOSTRIBIUM-BUTYRICUM DSM-5431, Letters in applied microbiology, 22(4), 1996, pp. 311-314
Citations number
15
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
02668254
Volume
22
Issue
4
Year of publication
1996
Pages
311 - 314
Database
ISI
SICI code
0266-8254(1996)22:4<311:GDA-TL>2.0.ZU;2-L
Abstract
Glycerol catabolism by Clostridium butyricum DSM 5431 into acetate, bu tyrate and 1,3-propanediol (1,3-PD) was studied in chemostat culture. The fact that the intracellular concentrations of NADH (18-22 mu mol g (-1) dry cell mass) mere extremely high suggested that the dehydratase activity was the rate limiting step in 1,3-PD formation. This limitat ion was proved by the addition of propionaldehyde, another substrate o f propanediol dehydrogenase, into the culture medium. This resulted in an increase in (i) glycerol utilization, (ii) biomass formation and ( iii) product biosynthesis.