THE UPTAKE AND FLOW OF C, N AND IONS BETWEEN ROOTS AND SHOOTS IN RICINUS-COMMUNIS L .4. FLOW AND METABOLISM OF INORGANIC NITROGEN AND MALATE DEPENDING ON NITROGEN NUTRITION AND SALT TREATMENT

Ricinus plants were supplied with nutrient solutions containing differ ent N-sources or different nitrate concentrations and were also expose d to mild salinity, Between 41 and 51 d after sowing, the ratio of ino rganic to total nitrogen in xylem and phloem saps, the content of inor ganic nitrogen and malate in tissues, and nitrate reductase activities were determined. The flows of nitrate, ammonium, and malate between r oot and shoot were modelled to identify the site(s) of inorganic nitro gen assimilation and to show the possible role of malate in a pH-stat mechanism, Only in the xylem of nitrate-fed plants did inorganic nitro gen, in the form of nitrate, play a role as the transport solute. The nitrate percentage of total nitrogen in the xylem sap generally increa sed in parallel with the external nitrate concentration. The contribut ion of the shoot to nitrate reduction increased with higher nitrate su pply. Under salt treatment relatively more nitrate was reduced in the root as compared with non-treated plants, Ammonium was almost totally assimilated in the root, with only a minor recycling via the phloem. N itrate reductase activities measured in vitro roughly matched, or were somewhat lower than, calculated rates of nitrate reduction, From the rates of nitrate reduction (OH--production) and rates of malate synthe sis (2H(+)-production) it was calculated that malate accumulation cont ributed 76, 45, or 39% to the pH-stat system during nitrate reduction in plants fed with 0.2, 1.0 or 4.0 mM nitrate, malate flow in the phlo em played no role, In tissues of ammonium-fed plants no malate accumul ation was found and malate flows in xylem and phloem were also relativ e low.