HUMAN-CHROMOSOME-1 LOCALIZATION OF THE GENE FOR A PROSTAGLANDIN-F2-ALPHA RECEPTOR-NEGATIVE REGULATORY PROTEIN

A protein that copurifies with the bovine prostaglandin F-2 alpha rece ptor (FP) has been isolated and the corresponding rat cDNA has been cl oned. Transfection experiments suggest that this protein inhibits bind ing of [H-3]prostaglandin F-2 alpha ([H-3]PGF(2 alpha)) to FP. Histolo gically, this protein (FP regulatory protein or FPRP) shows a distribu tion coinciding well with those cells and tissues that respond to PGF( 2 alpha). A portion of the 3' untranslated region of the human homolog to fprp was subcloned, sequenced, and oligonucleotide primers chosen that allow polymerase chain reaction (PCR) amplification specifically of the human fprp sequence. These primers were then used in a PCR-base d mapping protocol, The human fprp gene was first localized through hu man/rodent somatic cell hybrids to human chromosome 1 (100% concordanc e), and further through yeast artificial chromosome (YAC) pools to reg ion lp13.1-q21.3 (level 1 mapping). In view of the specific histologic localization of this negative regulator, possible pathological condit ions are mentioned that may cosegregate with this chromosomal region.