Jk. Choi et al., A MODIFIED COOMASSIE BLUE STAINING OF PROTEINS IN POLYACRYLAMIDE GELSWITH BISMARK-BROWN-R, Analytical biochemistry, 236(1), 1996, pp. 82-84
A rapid and sensitive protein staining method employing a mixed dye te
chnique has been developed. Solutions of Coomassie brilliant blue R-25
0 (CB, 0.2%) and Bismark brown R (BBR, 0.05%) were mixed in the volume
ratio of 1:0.75 for staining (final molar ratio, 4.5:1). In this stai
ning, BBR inhibits the binding of CB to gel matrix by forming ion-pair
ing complex with excess CB; therefore, it reduces staining/destaining
times and also enhances the staining effect of CB on protein band. As
a result, the mixed dye staining enables complete staining/destaining
within 20/25 min and detection of up to 25 ng of bovine serum albumin.
(C) 1996 Academic Press, Inc.