TRANSFORMATION OF THE EXTREMELY THERMOACIDOPHILIC ARCHAEON SULFOLOBUS-SOLFATARICUS VIA A SELF-SPREADING VECTOR

Authors
ELFERINK MGL SCHLEPER C ZILLIG W
Citation
Mgl. Elferink et al., TRANSFORMATION OF THE EXTREMELY THERMOACIDOPHILIC ARCHAEON SULFOLOBUS-SOLFATARICUS VIA A SELF-SPREADING VECTOR, FEMS microbiology letters, 137(1), 1996, pp. 31-35
Citations number
18
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
137
Issue
1
Year of publication
1996
Pages
31 - 35
Database
ISI
SICI code
0378-1097(1996)137:1<31:TOTETA>2.0.ZU;2-S
Abstract
We describe a transformation system for extremely thermophilic archaea of the genus Sulfolobus in the kingdom Crenarchaeota. We have constru cted in vitro a recombinant derivative of the recently described conju gative plasmid pNOB8, containing a beta-galactosidase gene downstream of a strong promotor. Transformation of a beta-galactosidase negative mutant b of Sulfolobus solfataricus with this construct resulted in it s spreading through the culture containing the primary transformants a nd in efficient restoration of beta-galactosidase activity