ISOLATION AND CHARACTERIZATION OF A MUTANT OF SACCHAROMYCES-CEREVISIAE AFFECTED IN THE FLO1 LOCUS

Authors
REBOREDO NM SIEIRO C BLANCO P VILLA TG
Citation
Nm. Reboredo et al., ISOLATION AND CHARACTERIZATION OF A MUTANT OF SACCHAROMYCES-CEREVISIAE AFFECTED IN THE FLO1 LOCUS, FEMS microbiology letters, 137(1), 1996, pp. 57-61
Citations number
18
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
137
Issue
1
Year of publication
1996
Pages
57 - 61
Database
ISI
SICI code
0378-1097(1996)137:1<57:IACOAM>2.0.ZU;2-2
Abstract
A non-flocculent strain of Saccharomyces cerevisiae was selected after EMS mutation of a flocculent and heterozygous FLOl locus diploid. The analysis of 25 asci from this diploid showed in all cases segregation 0F:4NF, thus confirming that it was probably affected in the desired gene. After sporulation and dissection of asci, three haploid strains were chosen, which were altered in the locus FLOl. Crossing these thre e strains with two other ones having markers for ADEl and pholl::LEU2, we could map the mutation at ca. 4.3 cM and ca. 37.7 cM from the PHOl l and ADEl loci respectively.