J. Gasperik et al., GROWTH-FACTORS ENCODED BY VARIOUS HERPESVIRUSES CAN BE RESOLVED IN 2-COMPONENT OR IN 3-COMPONENT, Biologisches Zentralblatt, 115(1), 1996, pp. 71-77
Three human and six animal herpesviruses were shown to produce virus-r
elated growth factors. All these factors similary to previously descri
bed pseudorabies virus growth factor (PRGF) induced a transformed phen
otype in normal MK-2 cells and repressed the transformed phenotype of
Hep-2 and T 24 tumor cells. By the use of gel filtration chromatograph
y on the FPLC Superose -12 column, seven growth factors could be resol
ved into, two and three factors were resolved in three small active co
mponents (Mr < 1000). Each of two components of the factors belonging
to the first group possessed only transforming activity, the transform
ed phenotype repressing activity could be reconstituted by simultaneou
s application of both components. The middle component of three-compon
ent factors possessed only transformation repressed activity, whereas
other two components were transforming only. The middle transformation
repressing components, devoid of transforming activity are discussed
as possible anticancerogenic substances.