S. Woodward et Rj. Thomson, MICROPROPAGATION OF THE SILK TASSEL BUSH, GARRYA-ELLIPTICA DOUGL, Plant cell, tissue and organ culture, 44(1), 1996, pp. 31-35
Proliferating cultures of Garrya elliptica were established from shoot
tip and nodal explants grown on woody plant medium containing benzyla
denine. Optimum numbers of healthy shoots were induced on media contai
ning 1.25 mu M benzyladenine; higher concentrations caused more hyperh
ydricity and less extension. Following growth for 28 days on quarter-s
trength woody plant medium containing 0.2% activated charcoal, 90% of
shoots rooted on the same medium lacking charcoal, but with 2.5 mu M i
ndolebutyric acid. Survival during weaning was poor, and was strongly
correlated with the time of year at which plants were removed from cul
ture.