PURIFICATION AND CHARACTERIZATION OF MICROBIAL GELLAN LYASE

Authors
HASHIMOTO W INOSE T NAKAJIMA H SATO N KIMURA S MURATA K
Citation
W. Hashimoto et al., PURIFICATION AND CHARACTERIZATION OF MICROBIAL GELLAN LYASE, Applied and environmental microbiology, 62(4), 1996, pp. 1475-1477
Citations number
11
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
62
Issue
4
Year of publication
1996
Pages
1475 - 1477
Database
ISI
SICI code
0099-2240(1996)62:4<1475:PACOMG>2.0.ZU;2-0
Abstract
Gellan lyase was purified from the culture fluid of soil samples incub ated in a medium containing gellan as a sole carbon source. The enzyme was a monomer with a molecular mass of 140 kDa and was most active at pH 7.5 and 45 degrees C. The enzyme was highly specific to gellan and lowered the viscosity of the polymer.