RAPID AND SENSITIVE METHOD FOR THE DETECTION OF MYCOBACTERIUM-CHLOROPHENOLICUM PCP-1 IN SOIL BASED ON 16S RIBOSOMAL-RNA GENE-TARGETED PCR

Authors
BRIGLIA M EGGEN RIL DEVOS WM VANELSAS JD
Citation
M. Briglia et al., RAPID AND SENSITIVE METHOD FOR THE DETECTION OF MYCOBACTERIUM-CHLOROPHENOLICUM PCP-1 IN SOIL BASED ON 16S RIBOSOMAL-RNA GENE-TARGETED PCR, Applied and environmental microbiology, 62(4), 1996, pp. 1478-1480
Citations number
17
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
62
Issue
4
Year of publication
1996
Pages
1478 - 1480
Database
ISI
SICI code
0099-2240(1996)62:4<1478:RASMFT>2.0.ZU;2-R
Abstract
A method based on 16S rRNA gene-targeted PCR and oligonucleotide probi ng was developed for detecting Mycobacterium chlorophenolicum PCP-1 in soil. The primers and probe were specific for PCP-1 in DNA extracts o f three soils. The method allowed for PCP-1 detection in soil with a d etection limit of 3 x 10(2) cells per g.