RAPID FLUORESCENCE ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR SUBTILISIN

A simple and rapid ELISA with a fluorescent end-point has been develop ed for the measurement of subtilisin. The ELISA can be applied to the analysis of enzyme in solutions that have been eluted from filters fol lowing the capture of air samples in the workplace. The assay has adeq uate precision and shows no interference from other enzymes tested. A comparison has been made between this assay and a similar ELISA method using spectrophotometric detection, The use of fluorescence defection reduces the over-all assay time from 60 to 25 min and reduces the con centrations of antibody reagents required in the assay. The assay desc ribed is quicker and more sensitive than the current methods in use.