CONVERSION OF STARCH TO ETHANOL IN A RECOMBINANT SACCHAROMYCES-CEREVISIAE STRAIN EXPRESSING RICE ALPHA-AMYLASE FROM A NOVEL PICHIA-PASTORISALCOHOL OXIDASE PROMOTER

Authors
KUMAGAI MH SVERLOW GG DELLACIOPPA G GRILL LK
Citation
Mh. Kumagai et al., CONVERSION OF STARCH TO ETHANOL IN A RECOMBINANT SACCHAROMYCES-CEREVISIAE STRAIN EXPRESSING RICE ALPHA-AMYLASE FROM A NOVEL PICHIA-PASTORISALCOHOL OXIDASE PROMOTER, Bio/technology, 11(5), 1993, pp. 606-610
Citations number
45
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Bio/technology → ACNP
ISSN journal
0733222X
Volume
11
Issue
5
Year of publication
1993
Pages
606 - 610
Database
ISI
SICI code
0733-222X(1993)11:5<606:COSTEI>2.0.ZU;2-L
Abstract
A recombinant Saccharomyces cerevisiae, expressing and secreting rice alpha-amylase, converts starch to ethanol. The rice alpha-amylase gene (OS103) was placed under the transcriptional control of the promoter from a newly described Pichia pastoris alcohol oxidase genomic clone. The nucleotide sequences of ZZA1 and other methanol-regulated promoter s were analyzed. A highly conserved sequence (TTG-N3-GCTTCCAA-N5-TGGT) was found in the 5' flanking regions of alcohol oxidase, methanol oxi dase, and dihydroxyacetone synthase genes in Pichia pastoris, Hansenul a polymorpha, and Candida boidinii S2. The yeast strain containing the ZZA1-OS103 fusion secreted biologically active enzyme into the cultur e media while fermenting soluble starch.