RAPID AND SENSITIVE DETERMINATION OF PIROXICAM IN RAT PLASMA, MUSCLE AND SKIN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
CERRETANI D MICHELI L FIASCHI AI GIORGI G
Citation
D. Cerretani et al., RAPID AND SENSITIVE DETERMINATION OF PIROXICAM IN RAT PLASMA, MUSCLE AND SKIN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography. Biomedical applications, 614(1), 1993, pp. 103-108
Citations number
13
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography. Biomedical applications → ACNP
ISSN journal
03784347
Volume
614
Issue
1
Year of publication
1993
Pages
103 - 108
Database
ISI
SICI code
0378-4347(1993)614:1<103:RASDOP>2.0.ZU;2-A
Abstract
A rapid and precise high-performance liquid chromatographic method for the determination of piroxicam in a variety of biological samples has been developed. A reversed-phase column, isocratic elution and ultrav iolet detection were employed. Calibration curves were reproducible an d highly linear, with correlation coefficients typically averaging ove r 0.992. The detection limit of the assay was 100 ng/ml for all biolog ical samples examined (at a signal-to-noise ratio of 3:1). Validation of the method demonstrated a good sensitivity, accuracy and precision. The method has been adopted for a pharmacokinetic study in rats.