SIMPLE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR POLYAMINES AND THEIR MONOACETYL DERIVATIVES

Authors
TAIBI G SCHIAVO MR
Citation
G. Taibi et Mr. Schiavo, SIMPLE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR POLYAMINES AND THEIR MONOACETYL DERIVATIVES, Journal of chromatography. Biomedical applications, 614(1), 1993, pp. 153-158
Citations number
24
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography. Biomedical applications → ACNP
ISSN journal
03784347
Volume
614
Issue
1
Year of publication
1993
Pages
153 - 158
Database
ISI
SICI code
0378-4347(1993)614:1<153:SHLAFP>2.0.ZU;2-Z
Abstract
A rapid reversed-phase high-performance liquid chromatographic method, using pre-column derivatization with benzoyl chloride and ultraviolet detection at 254 nm, was developed for the simultaneous measurement o f polyamines and their monoacetyl derivatives. Calibration curves were linear for concentrations from 1.25 to 25 nmol/ml. The method was emp loyed to assay these compounds in chick embryo retina explants using o rganic solvent extraction and 1,7-diaminoheptane as an internal standa rd. This simple and sensitive method can be applied to routine determi nations of these compounds in various biological samples.